Anti-ADAM15 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q13444

Core Product Specifications and Parameters

Biological Background: ADAM15 Function and Localization

• ADAM15, also known as MDC15 or metargidin, is a member of the ADAM (a disintegrin and metalloproteinase) family, possessing metalloprotease, disintegrin, EGF-like, and cysteine-rich domains; multiple isoforms are generated by alternative splicing, reflected in variable molecular weights (55–93 kDa).
• It functions as an active metalloproteinase with gelatinolytic and collagenolytic activities, contributing to extracellular matrix remodeling during wound healing and angiogenesis.
• ADAM15 mediates heterotypic intraepithelial cell/T‑cell interactions and homotypic T‑cell aggregation, and inhibits integrin‑mediated cell adhesion and migration in airway smooth muscle cells.
• The protein is involved in pathological neovascularization and cartilage remodeling, and its disintegrin domain may participate in sperm‑egg binding during fertilization.
• Subcellularly, ADAM15 localizes to the endomembrane system, adherens junctions, cilia, flagella, and acrosomal vesicles, consistent with its roles in adhesion and proteolysis.
• It is ubiquitously expressed, with prominent levels in colon, small intestine, airway smooth muscle, and glomerular mesangial cells, and is overexpressed in atherosclerotic lesions.
• Post‑translational processing includes cleavage at dibasic sites (zymogen activation), glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen comprises a synthetic peptide within the metalloprotease domain (amino acids 200–300), so the antibody may detect both pro‑ and active forms; consider using positive/negative controls (e.g., knockdown or knockout) to confirm specificity.
• For Western blotting, multiple bands corresponding to isoforms (55–93 kDa) are expected; validate band identity with isoform‑specific siRNAs or overexpression.
• When performing IF‑P or IHC‑P, optimize antigen retrieval and antibody dilution for the target tissue; cross‑reactivity with mouse and rat suggests conserved epitopes, but validation in the relevant sample system is recommended.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials. For ADAM15‑related projects—whether investigating cell adhesion, proteolysis, or angiogenesis—we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our team also assists with rare‑target raw‑material sourcing to help accelerate your development from concept to clinic.