Anti-ADAM12 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O43184

Core Product Specifications and Parameters

Biological Background: ADAM12 Function and Localization

• ADAM12, also known as Meltrin-alpha, is a member of the ADAM (a disintegrin and metalloproteinase) family of proteins.
• It plays a key role in skeletal muscle regeneration by promoting cell fusion at the onset of myoblast fusion.
• ADAM12 contributes to the formation of macrophage-derived giant cells and osteoclasts from mononuclear precursors.
• The protein is localized at the cell membrane and is also secreted into the extracellular space.
• Two major isoforms exist: isoform 1 is expressed in placenta, skeletal, cardiac, and smooth muscle; isoform 2 is restricted to placenta, embryo, and fetal tissues, with both detected in some tumor cell lines.
• ADAM12 contains multiple structural domains, including a metalloprotease domain, a disintegrin domain, and an EGF-like domain, and requires zinc for its catalytic activity.
• Post-translational modifications include glycosylation, phosphorylation, and cleavage on basic residues, which regulate its activation and function.

Experimental Guidance and Technical Tips

• For Western blot, a band around 100 kDa is expected; reducing conditions may be needed to observe the mature cleaved form.
• Muscle tissue lysates (skeletal or cardiac) are appropriate positive controls given the tissue distribution.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval may be required; optimization of antibody dilution is recommended.
• In immunofluorescence (IF/ICC), permeabilization protocols should be validated for the specific cell type to ensure access to the intracellular and membrane-bound pools of ADAM12.
• Cross-reactivity with mouse and rat enables application in common rodent models; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio facilitates the sourcing of high-quality IVD raw materials, including validated polyclonal antibodies like this anti-ADAM12 antibody, for diagnostic and research applications in muscle biology and cell fusion studies. Our supply capabilities encompass antibody pairs, recombinant proteins, and ancillary reagents to support your assay development and manufacturing needs.