Anti-ADAM11 Polyclonal Antibody for WB, IF/ICC, ELISA - O75078

Core Product Specifications and Parameters

Biological Background: ADAM11 Function and Localization

• ADAM11 (Disintegrin and metalloproteinase domain-containing protein 11), also known as MDC, is a transmembrane protein belonging to the ADAM family that lacks catalytic metalloprotease activity due to mutations in the zinc-binding motif.
• It functions as a probable ligand for integrins in the brain and is critical for proper localization of potassium channel subunits KCNA1/KV1.1 and KCNA2/KV1.2 at cerebellar basket cell distal terminals, thereby facilitating ephaptic inhibitory synchronization of Purkinje cell firing and modulation of stress responses.
• ADAM11 is involved in spatial learning, motor coordination, and the nociceptive pain response to chemical stimulation, likely through its role in synaptic organization (by similarity).
• The protein is predominantly expressed in the brain, with little to no expression in other tissues, consistent with its neuronal functions.
• Subcellularly, ADAM11 localizes to the presynaptic cell membrane, perikaryon, and axonal projections, underscoring its role at neuronal synapses.
• ADAM11 undergoes alternative splicing, is glycosylated, contains an EGF-like domain, and has multiple disulfide bonds; its sequence includes a signal peptide, a transmembrane helix, and pairs of basic residues that may be cleaved.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 290–430 within the extracellular region of human ADAM11, which may contribute to the antibody's specificity for detecting the native protein across multiple applications such as WB, IF/ICC, and ELISA.
• For Western blot, the predicted molecular weight is approximately 83 kDa (calculated 83.4 kDa from sequence). Bands near this size are expected in brain tissue lysates; confirm identity using appropriate controls.
• In immunofluorescence/ICC, consider that ADAM11 is enriched in neuronal cells and localizes to presynaptic membranes and axons; appropriate fixation and permeabilization conditions should be optimized.
• The antibody is reported to cross-react with mouse and rat ADAM11, making it suitable for comparative studies across common rodent models; validate in the relevant sample system before quantitative analyses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials. This anti-ADAM11 polyclonal antibody supports neuroscience and neurodevelopmental studies, demonstrating our commitment to supplying validated antibodies for challenging membrane proteins. Beyond individual reagents, we offer one-stop sourcing of antibody pairs, bulk ancillary reagents, and custom procurement for rare or emerging targets.