Anti-ACY1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q03154

Core Product Specifications and Parameters

Biological Background: ACY1 Function and Localization

• ACY1 (Aminoacylase-1, also known as N-acyl-L-amino-acid amidohydrolase; aliases ACY-1, ACY1D, HEL-S-5) is a zinc-dependent hydrolase encoded by the ACY1 gene on chromosome 3.
• It catalyzes the hydrolysis of N‑acetylated and N‑formylated amino acids into free amino acids and carboxylates, playing a key role in the degradation of N-acylated peptides.
• ACY1 may act sequentially with acylaminoacyl-peptidase (APEH): APEH first releases N-acylamino acids from peptides, after which ACY1 cleaves the amide bond.
• The protein localizes to the cytoplasm and is expressed at high levels in kidney, strongly in brain, and at lower levels in placenta and spleen.
• Metal-binding is essential: ACY1 coordinates zinc ions, which are required for its enzymatic activity.
• Alternative splicing generates multiple transcript variants; mutations in ACY1 are linked to aminoacylase-1 deficiency, a metabolic disorder.
• The enzyme belongs to the M20 metallopeptidase family and has been identified in proteomics studies as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen covers full‑length human ACY1 (aa 1‑408); verify that the antibody recognizes the expected isoform(s) and any cross‑reactive species (mouse/rat) by initial test blots or pilot IHC.
• For WB, expect a band at ~46 kDa; include a loading control and use tissue lysates with high ACY1 expression (e.g., kidney, brain) as positive controls.
• For IHC‑P, optimize antigen retrieval and consider counterstaining; strong cytoplasmic staining is anticipated in renal tubular epithelium and neuronal populations.
• For IF/ICC, the anticipated pattern is cytoplasmic; nuclear counterstain (DAPI) will help confirm subcellular localization.
• For ELISA, the antibody may serve as either capture or detection reagent; validate sensitivity and specificity in the sample matrix of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. This anti‑ACY1 polyclonal antibody aligns with research into amino acid metabolism, renal physiology, and inherited metabolic disorders, and CamelBio can support your workflow from concept to clinic with complementary reagents and technical consultation. For one‑stop procurement of optimized antibodies and critical assay components, our team is ready to assist your project requirements.