Anti-ACY1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q03154

Core Product Specifications and Parameters

Biological Background: ACY1 Function and Localization

• ACY1 (Aminoacylase-1), also known as N-acyl-L-amino-acid amidohydrolase, primarily hydrolyzes N-acetylated and N-formylated amino acids into free amino acids and carboxylate.
• It may sequentially cooperate with APEH in the degradation of N-acylated peptides: APEH releases N-acylamino acids, which are then cleaved by ACY1.
• The enzyme localizes to the cytoplasm.
• Tissue expression is highest in kidney, strong in brain, and weaker in placenta and spleen.
• ACY1 is a zinc-binding metalloenzyme, requiring zinc ions for catalytic activity.
• Alternative splicing generates multiple transcript variants.
• The protein is associated with disease; mutations lead to aminoacylase-1 deficiency.
• Its three-dimensional structure has been determined, and it is identified in proteomics screens.

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against full-length recombinant human ACY1 (aa 1-408), is suitable for detecting ACY1 in Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA.
• For WB, the predicted molecular weight is ~46 kDa; validate band specificity in relevant cell or tissue lysates (kidney, brain).
• In IF/ICC, permeabilization is required to access cytoplasmic ACY1; optimize fixation and permeabilization protocols for best signal.
• For ELISA, consider using the antibody as a capture or detection reagent after validating sensitivity and specificity with recombinant ACY1 or cell lysates.
• Cross-reactivity with mouse and rat ACY1 has been shown, enabling studies across species; verify in specific experimental systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, from validated antibodies and antibody pairs to bulk reagents and rare target sourcing. For metabolic and amino acid degradation research, CamelBio’s ACY1 antibody supports diagnostic development in renal and neurological applications. Partner with us to streamline your assay development from concept to clinic.