Anti-ACVR2B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13705

Core Product Specifications and Parameters

Biological Background: ACVR2B Function and Localization

• Encoded by ACVR2B, activin receptor type-2B is a transmembrane serine/threonine kinase that functions as the primary receptor for activins and inhibins, containing extracellular ligand-binding, transmembrane, and intracellular kinase domains.
• As a type-2 receptor, ACVR2B forms heteromeric complexes with type-1 receptors (ACVR1, ACVR1B, ACVR1c) upon ligand binding, initiating downstream signaling cascades critical for numerous biological processes.
• Activin binding activates the kinase domain, which phosphorylates type-1 receptors; these in turn phosphorylate SMAD2/3, leading to SMAD4 complex formation and nuclear translocation to regulate gene transcription (e.g., neuronal differentiation, FSH production, wound healing).
• Signaling is antagonized by inhibin-B binding through the IGSF1 coreceptor and by inhibitory SMAD7, which blocks SMAD2/3 association with the receptor complex.
• Localized to the cell membrane, ACVR2B contains disulfide bonds and is glycosylated; its molecular weight is approximately 57.7 kDa (UniProt), with a reported protein weight of 58 kDa.
• Keywords: alternative splicing, disease variant (associated with heterotaxy), ATP-binding, and reference proteome; these highlight structural complexity and clinical relevance in developmental disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 50-150 of human ACVR2B (extracellular domain), suggesting recognition of the native and denatured protein.
• For Western blot (WB), consider using reducing and denaturing conditions; a band at ~58 kDa is expected. Endogenous ACVR2B may be detected in relevant cell lines, though expression levels may require optimization.
• For immunofluorescence (IF/ICC), permeabilization is likely needed to access the intracellular domain; validate antibody specificity with appropriate controls.
• For ELISA, the peptide-based immunogen supports detection; titrate antibody concentration and use a matched coating buffer for optimal performance.
• Cross-reactivity is reported for human ACVR2B; sequence homology with other species is not specified, so validate in non-human systems if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering this anti-ACVR2B rabbit polyclonal antibody alongside a wide range of validated antibody pairs, recombinant proteins, and ancillary reagents. We support diagnostic manufacturers and research laboratories in activin signaling and developmental biology studies with reliable, high-quality materials. From concept to clinic, our supply capabilities cover bulk orders, custom antibody development, and rare-target sourcing to accelerate your assay development and commercialization.