Anti-ACVR2A Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P27037

Core Product Specifications and Parameters

Biological Background: ACVR2A Function and Localization

• ACVR2A (Activin receptor type-2A), also known as ACTR-IIA or ACTRIIA, is a transmembrane serine/threonine kinase that belongs to the transforming growth factor-beta (TGF-β) receptor family.
• It functions as a type II receptor for activin A, activin B, and inhibin A. Upon ligand binding, it forms a heteromeric complex with type I receptors, leading to phosphorylation and activation of the type I receptors, which then propagate signals through SMAD transcriptional regulators. Related references: PMID:17911401 PMID:10652306
• ACVR2A mediates the induction of adipogenesis by GDF6, highlighting its role in cellular differentiation processes (by similarity).
• The protein is localized at the cell membrane, where it acts as a signal transducer, and its extracellular domain contains the ligand-binding region, while the intracellular domain possesses kinase activity.
• Post-translational modifications include glycosylation and disulfide bond formation, which are important for proper folding and function. ACVR2A is also subject to alternative splicing, potentially generating isoforms with distinct regulatory properties.
• Key structural features include an ATP-binding site, a kinase domain, and a single transmembrane helix, with the receptor requiring metal ions such as manganese or magnesium for its enzymatic activity.

Experimental Guidance and Technical Tips

• The immunogen used covers amino acids 20-140 of the extracellular domain, which is expected to generate antibodies recognizing the ligand-binding region; consider this when designing epitope-relevant assays.
• For Western blot (WB), a band around 58 kDa is predicted; however, glycosylation may cause slight migration shifts, so validate in your sample system.
• The antibody is reported to react with human, mouse, and rat species; when using mouse or rat tissues, perform appropriate positive and negative controls to confirm specificity.
• For immunofluorescence/immunocytochemistry (IF/ICC) and immunohistochemistry (IHC-P), cell membrane localization is expected; optimize fixation and permeabilization conditions to preserve epitopes.
• In ELISA applications, pairing with a capture or detection antibody against a non-competing epitope may improve assay performance; test different concentrations to establish optimal conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers exploring TGF-β/activin signaling and receptor biology, we offer this ACVR2A polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing capabilities. Whether your project requires consistent supply or specialized reagents, CamelBio is your trusted B2B partner for IVD raw materials.