Anti-ACVR1B Rabbit Polyclonal Antibody for WB, ELISA - P36896

Core Product Specifications and Parameters

Biological Background: ACVR1B Function and Localization

• ACVR1B, also known as activin receptor-like kinase 4 (ALK-4) or SKR2, is a transmembrane serine/threonine kinase that functions as a type-1 receptor for activins, mediating signaling in the TGF-β superfamily.
• Upon ligand binding, ACVR1B forms a heteromeric complex with type-2 activin receptors (ACVR2A or ACVR2B), leading to phosphorylation and activation of its kinase domain. The activated receptor then phosphorylates downstream SMAD2 and SMAD3 proteins, which complex with SMAD4 to regulate target gene transcription.
• ACVR1B signaling is modulated by inhibitory SMAD7, which is recruited via FKBP1A to block SMAD2/3 association, and by the inhibin-B coreceptor IGSF1.
• Alternative splicing generates multiple isoforms of ACVR1B.
• The receptor localizes to the cell membrane and is expressed in many tissues, with strongest expression in kidney, pancreas, brain, lung, and liver.
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitination, which may regulate its activity and stability.
• ACVR1B plays critical roles in diverse processes including neuronal differentiation, hair follicle cycling, FSH production, wound healing, extracellular matrix production, and carcinogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 24-126) of human ACVR1B, which may favor detection of the full-length receptor under non-denaturing conditions or in ELISA formats.
• A specific band at ~57 kDa is expected in Western blot using human or mouse lysates from ACVR1B-expressing tissues (e.g., kidney, pancreas).
• For ELISA applications, consider coating with recombinant ACVR1B extracellular domain and using this antibody as a capture or detection reagent; pairing with a matched monoclonal may improve sensitivity.
• Validate antibody specificity in relevant cellular models and tissue samples, as ACVR1B is broadly expressed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibodies like this ACVR1B polyclonal antibody for research in activin signaling and TGF-β pathways. We supply optimized antibody pairs, bulk ancillary reagents, and support rare target raw-material procurement to streamline your diagnostic development.