Anti-ACTR1B Monoclonal Antibody for WB, IHC-P, IP, ELISA - P42025

Core Product Specifications and Parameters

Biological Background: Beta-centractin Function and Localization

• Beta-centractin (also known as actin-related protein 1B, ARP1B, or CTRN2) is encoded by the ACTR1B gene and has a predicted molecular weight of approximately 42 kDa.
• It is a component of a multi-subunit complex that participates in microtubule-based vesicle motility.
• This protein is associated with the centrosome, the primary microtubule-organizing center of the cell.
• Beta-centractin localizes to the cytoplasm and cytoskeleton, with specific enrichment at the centrosome.
• It belongs to the actin family of proteins, characterized by ATP and nucleotide binding.
• Post-translational modifications of beta-centractin include acetylation and nitration, which may modulate its activity.
• The protein has been identified by direct protein sequencing and in high-throughput proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 300–376) of human ACTR1B, which may provide specific recognition of the full-length protein. Confirm reactivity in the relevant sample system and consider using knockout controls to verify specificity.
• For Western blotting, a band around 42 kDa is expected in human and rat lysates. Ensure appropriate loading controls and molecular weight markers are included.
• In immunohistochemistry (paraffin sections), optimize antigen retrieval and fixation conditions, as centrosomal localization requires careful preservation of cellular architecture.
• For immunoprecipitation, use a validated lysis buffer that maintains protein complex integrity, as ACTR1B interacts with dynactin components. Include an isotype control to assess non-specific binding.
• When performing ELISA, titrate the antibody against the recombinant immunogen or cell lysates and establish a standard curve for quantitative analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw-material sourcing, providing a complete portfolio of high-quality antibodies, antigens, and ancillary reagents to support diagnostic development from concept to clinic. This anti-ACTR1B monoclonal antibody is one example of our specialized offerings for cytoskeleton and cell biology research. Whether you need validated antibody pairs, optimized monoclonals, or bulk reagents for large-scale production, CamelBio streamlines your supply chain with reliable, one-stop access.