Anti-ACTR1A Rabbit Polyclonal Antibody for WB, ELISA - P61163

Core Product Specifications and Parameters

Biological Background: Alpha-centractin Function and Localization

• Alpha-centractin (ACTR1A) is also known as ARP1, Actin-RPV, and centrosome-associated actin homolog, a member of the actin-related protein family.
• ACTR1A forms a filament together with ACTB that serves as the structural backbone for the dynactin complex.
• The dynactin complex is critical for activating the molecular motor dynein, enabling ultra-processive transport of cargo along microtubules.
• ACTR1A localizes to the cytoplasm, cytoskeleton, microtubule organizing center (centrosome), and cell cortex.
• The protein contains ATP-binding and nucleotide-binding domains, consistent with its actin-like properties.
• Post-translational acetylation has been identified, and the protein is a reference proteome member with direct protein sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 187-376 of human ACTR1A, which may confer specificity for detection across human, mouse, and rat species as indicated.
• For Western blot (WB), consider using a range of lysate concentrations from relevant cell lines or tissues to optimize signal with the predicted band at ~43 kDa.
• In ELISA, it is advisable to validate the antibody’s reactivity with the target protein under your specific assay conditions, as performance may vary with coating buffers and detection systems.
• Given the involvement of ACTR1A in the dynactin complex, co-immunoprecipitation experiments might be considered to study protein interactions, but require separate validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your dynactin and cytoskeleton research by offering high-quality anti-ACTR1A antibodies, validated antibody pairs, and ancillary reagents. Our one-stop platform simplifies sourcing of IVD raw materials from early exploration to clinical application, ensuring reliable supply for your pipeline.