Anti-ACTN3 Polyclonal Antibody for WB, IHC-P, ELISA - Q08043

Core Product Specifications and Parameters

Biological Background: ACTN3 Function and Localization

• Alpha-actinin-3, also known as ACTN3 or alpha-actinin skeletal muscle isoform 3, is an F-actin cross-linking protein encoded by the ACTN3 gene.
• It functions as an actin-bundling protein that anchors actin filaments to various intracellular structures, contributing to the maintenance and regulation of the sarcomeric cytoskeleton.
• Expression is restricted to fast (type 2) skeletal muscle fibers at the protein level, highlighting its role in rapid contraction and muscle performance.
• The protein belongs to the alpha-actinin family and contains multiple spectrin repeats, an actin-binding domain, and an EF-hand calcium-binding domain (inferred from keywords).
• Key post-translational modifications include acetylation, and calcium binding is essential for its structural and functional modulation.
• The protein’s 3D structure has been solved, and it has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 580–630 of human ACTN3, a conserved region across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, expect a band at approximately 103 kDa; consider using muscle tissue lysates enriched in fast-twitch fibers to confirm specificity.
• For immunohistochemistry (IHC-P), evaluate staining on skeletal muscle sections, where ACTN3 is expressed in type 2 fibers.
• ELISA may be used with purified protein or peptides; validate performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For muscle biology and neuromuscular disorder research, our sourcing portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Whether you need rare-target raw-material sourcing or complete assay development support, CamelBio is your trusted partner.