Anti-ACTN3 Monoclonal Antibody for WB, IF-P, ELISA - Q08043

Core Product Specifications and Parameters

Biological Background: Alpha-actinin-3 Function and Localization

• Alpha-actinin-3 (ACTN3) is an F-actin cross-linking protein that bundles actin filaments and anchors them to intracellular structures.
• It is specifically expressed in fast (type 2) skeletal muscle fibers (at protein level).
• The protein is a member of the alpha-actinin family, characterized by spectrin repeats and EF-hand calcium-binding domains.
• Key biological roles include actin-binding and calcium-dependent regulation, contributing to muscle contraction and cytoskeletal organization.
• Post-translational modifications include acetylation.
• The 3D structure of ACTN3 has been determined.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 700-800 within the C-terminal EF-hand domain of human ACTN3, potentially conferring isoform specificity; consider this when designing experiments.
• For Western blot (WB), validate in fast skeletal muscle lysates where ACTN3 is enriched; the predicted molecular weight is ~103 kDa.
• In immunofluorescence (IF-P), optimize fixation and permeabilization protocols to preserve actin-rich structures where ACTN3 localizes.
• ELISA applications require careful optimization of coating and detection antibodies; include appropriate controls and validate in the relevant sample system.
• Observed cross-reactivity with mouse and rat allows translational studies, but always verify target specificity in your model organism.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and researchers with streamlined access to validated antibodies, recombinant proteins, and ancillary reagents for muscle biology and beyond. This anti-ACTN3 monoclonal antibody exemplifies our catalog of skeletal muscle markers, available in bulk with consistent quality. Our sourcing team also specializes in rare target raw materials, helping you bridge the gap from concept to clinic efficiently.