Anti-ACTN2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P35609

Core Product Specifications and Parameters

Biological Background: Alpha-actinin-2 Function and Localization

• Alpha-actinin-2 (ACTN2) is a cytoskeletal protein that belongs to the spectrin gene family and functions as an F-actin cross-linking and bundling protein.
• It anchors actin filaments to various intracellular structures, playing a critical role in maintaining sarcomere integrity and muscle contraction.
• ACTN2 is specifically expressed in both skeletal and cardiac muscle tissues.
• Subcellularly, ACTN2 localizes to the cytoplasm, myofibril, and sarcomere Z line, where it cross-links actin filaments.
• Alternative splicing yields multiple isoforms, contributing to functional diversity in muscle cells.
• The protein contains calcium-binding and actin-binding domains, and its activity may be regulated by phosphorylation and ubiquitination.
• Mutations in ACTN2 are associated with cardiomyopathies, including dilated and hypertrophic cardiomyopathy, highlighting its essential role in cardiac muscle function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–250) of human ACTN2; consider sequence alignment if exploring cross-reactivity with other species beyond those listed.
• For Western blot, empirically optimize the dilution; the expected molecular weight is approximately 104 kDa.
• In immunofluorescence, validate fixation and permeabilization protocols to detect the characteristic Z-line pattern.
• For ELISA, this antibody can be used for antigen detection; consider pairing with a matched capture/detection antibody for sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research labs, offering one-stop access to validated antibody pairs, optimized polyclonal antibodies like this ACTN2 antibody, and bulk ancillary reagents. Specializing in cardiac and skeletal muscle biomarker development, we also provide rare target raw-material sourcing and technical consulting to support your project from concept to clinic.