Anti-ACTN2 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P35609

Core Product Specifications and Parameters

Biological Background: Alpha-actinin-2 Function and Localization

• Alpha-actinin-2 is an F-actin cross-linking protein that bundles actin filaments and anchors them to intracellular structures such as the sarcomere Z-line.
• Encoded by the ACTN2 gene, this protein belongs to the alpha-actinin family and is the skeletal muscle-specific isoform, also known as alpha-actinin skeletal muscle isoform 2.
• Alternative splicing produces multiple isoforms, with reported molecular weights around 104 kDa (UniProt: 103854 Da).
• Localizes to the cytoplasm, primarily to the myofibril sarcomere Z-line, where it cross-links actin filaments and helps maintain sarcomere integrity.
• Expressed predominantly in skeletal and cardiac muscle, aligning with its role in muscle contraction and mechanotransduction.
• Contains multiple spectrin repeats and an actin-binding domain, and is regulated by calcium binding, phosphorylation, and ubiquitination, as indicated by its keywords.
• Clinically, mutations in ACTN2 are associated with cardiomyopathies and myopathies, underscoring its importance in muscle disease.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit monoclonal antibody resides within the N-terminal region (aa 1–100) of human ACTN2, which is highly conserved; thus, cross-reactivity with mouse and rat is expected. Consider sequence alignment to verify conservation in other species.
• For Western blotting, the predicted molecular weight is 104 kDa; however, alternative splicing or post-translational modifications may yield additional bands. Validate with appropriate controls in your sample system.
• For immunofluorescence (IF-P), ACTN2 localizes to the Z-line of sarcomeres, resulting in a striated staining pattern in muscle cells. Co-staining with sarcomeric markers (e.g., myosin) may help confirm specificity.
• This antibody may be suitable for ELISA-based assays; however, experimental optimization of coating antigens and detection conditions is recommended.
• Use appropriate sample preparation, such as total protein lysates from cardiac or skeletal muscle tissue for WB, and fixed/permeabilized muscle cell lines or tissue sections for IF.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on sarcomere structure and muscle-related diseases, this anti-ACTN2 antibody supports investigations into cardiac and skeletal muscle function. We also specialize in sourcing hard-to-find raw materials to accelerate your assay development from concept to clinic.