Anti-ACTN1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P12814

Core Product Specifications and Parameters

Biological Background: ACTN1 Function and Localization

• ACTN1 encodes alpha-actinin-1, a 103 kDa non-muscle cytoskeletal isoform belonging to the spectrin family, characterized by N-terminal actin-binding domains and C-terminal calcium-binding EF-hand motifs.
• It functions as an F-actin cross-linking protein, bundling actin filaments and anchoring them to various intracellular structures, thereby maintaining cytoskeletal integrity and cell shape.
• Alpha-actinin-1 participates in immune synapse formation: its association with IGSF8 at the T-cell/antigen-presenting cell interface is required for efficient T-cell activation. Related references: PMID:22689882
• The protein localizes to diverse subcellular sites, including the Z-lines of sarcomeres, adhesion plaques, focal adhesions, and dynamic structures such as membrane ruffles and cell junctions.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its actin-binding affinity and subcellular targeting.
• Alternative splicing generates multiple isoforms with distinct tissue distributions and functional specializations.
• Mutations in ACTN1 are associated with inherited platelet disorders (e.g., BDPLT15), highlighting its critical role in megakaryocyte and platelet cytoskeletal organization.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein spanning amino acids 1–330, covering the N-terminal actin-binding domain; the antibody is expected to recognize total ACTN1 irrespective of isoform.
• Recommended for western blot (WB) detection of the ~103 kDa band in human and mouse cell or tissue lysates; consider using nuclear/cytoplasmic fractionation to enrich signals.
• For immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized; positive staining in muscle, platelets, and cultured cells can serve as controls.
• In ELISA, the antibody can function as a capture or detection reagent; pairing with another clone may improve sensitivity in sandwich formats.
• Cross-reactivity with human and mouse is confirmed; for other species, validate by sequence alignment of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying high-quality IVD raw materials, and this anti-ACTN1 antibody supports researchers and diagnostic developers focusing on cytoskeletal biology, platelet disorders, and T-cell activation. As a one-stop sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target procurement—backed by end-to-end technical and regulatory support to accelerate your product development pipeline.