Anti-ACTL6B Polyclonal Antibody (BAF53B) for WB/IHC/IF/ELISA - O94805

Core Product Specifications and Parameters

Biological Background: ACTL6B Function and Localization

• ACTL6B (Actin-like protein 6B), also known as BAF53B, ArpNalpha, is a 53 kDa BRG1-associated factor encoded by the ACTL6B gene.
• It is a core component of the SWI/SNF chromatin remodeling complex, which alters DNA-histone contacts in an ATP-dependent manner to regulate transcription.
• ACTL6B is specifically incorporated into the neuron-specific nBAF complex, replacing ACTL6A during neural differentiation, and is essential for postmitotic neural development and dendritic outgrowth.
• The nBAF complex, together with CREST, targets promoters of genes critical for dendritic growth; studies show ACTL6B is indispensable for this process (PubMed:31031012). Related references: PMID:31031012
• Localized predominantly in the nucleus, consistent with its chromatin regulatory function.
• Mutations in ACTL6B are associated with developmental epileptic encephalopathy and intellectual disability, highlighting its role in neurodevelopment.
• Tissue expression is neuronal; it is required for dendritic maturation in postmitotic neurons.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-280 amino acids of human ACTL6B; the resulting polyclonal antibody may recognize multiple epitopes within this region.
• For Western blotting, a band around 47 kDa (the predicted molecular weight) is expected in reduced samples; use appropriate molecular weight markers.
• In IHC-P and IF/ICC, expect nuclear staining; antigen retrieval may be required for formalin-fixed tissues.
• The antibody cross-reacts with mouse and rat ACTL6B, extending its utility to rodent models.
• For ELISA, consider pairing with a matched detection antibody if quantitative detection is needed, and validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic, laboratory, and research clients with integrated access to high-quality IVD raw materials like validated antibodies, bulk ancillary reagents, and custom antibody development. If you are researching chromatin remodeling or neurodevelopmental disorders, we can support your workflow with optimized polyclonal and monoclonal antibodies for key targets like ACTL6B. Our one-stop platform bridges the gap from concept to clinic, ensuring consistent supply and technical consultation for your assay development needs.