Anti-ACTL6A Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O96019

Core Product Specifications and Parameters

Biological Background: ACTL6A Function and Localization

• ACTL6A, also known as BAF53A, ArpNbeta, or INO80 complex subunit K, is an actin-like protein that serves as a core component of multiple chromatin-modifying complexes.
• It is a critical subunit of the SWI/SNF chromatin remodeling complex, enhancing the ATPase activity of SMARCA4/BRG1 to alter DNA-histone contacts and facilitate transcriptional regulation.
• In neural stem and progenitor cells, ACTL6A is essential for the npBAF complex, maintaining self-renewal and proliferation; its developmental switch with ACTL6B drives neuronal differentiation and dendrite growth.
• ACTL6A also participates in the NuA4 histone acetyltransferase (HAT) complex, acetylating histones H4 and H2A to promote gene activation, and in the INO80 complex, which regulates DNA replication and repair.
• The protein localizes to the nucleus and undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination (via isopeptide bonds).
• Genetic variations in ACTL6A have been associated with intellectual disability, consistent with its role in neurogenesis and chromatin regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 201–290 of human ACTL6A; consider verifying the epitope recognition in the relevant cell or tissue lysate.
• For Western blotting, the predicted molecular weight is 47 kDa; using nuclear-enriched lysates may improve signal detection.
• IHC-P applications may benefit from proper antigen retrieval optimization; validate staining in positive control tissues known to express ACTL6A.
• ELISA experiments may use recombinant full-length ACTL6A or its fragments as a positive control to set standard curves.
• Confirm cross-reactivity with mouse and rat samples experimentally, as reactivity is based on sequence homology.

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