Anti-Active Caspase-3 Monoclonal Antibody for WB, ELISA - P42574

Core Product Specifications and Parameters

Biological Background: Caspase-3 Function and Localization

• Caspase-3 is a thiol protease that acts as a major effector caspase in the execution phase of apoptosis, catalyzing the cleavage of numerous proteins. Related references: PMID:18723680, PMID:20566630, PMID:23650375
• At the onset of apoptosis, it cleaves poly(ADP-ribose) polymerase PARP1 at a specific aspartate-glycine bond. Related references: PMID:10497198, PMID:16374543, PMID:7596430
• Caspase-3 activates downstream caspases such as caspase-6, -7, and -9, amplifying the apoptotic signal. Related references: PMID:7596430
• It cleaves huntingtin, and is implicated in Huntington's disease pathology. Related references: PMID:8696339
• In addition to apoptosis, caspase-3 mediates pyroptosis by cleaving gasdermin-E (GSDME). Related references: PMID:35338844, PMID:35446120
• Caspase-3 is highly expressed in immune cells and tissues such as lung, spleen, and heart, and is predominantly cytoplasmic.
• The protein exists as a zymogen and is activated by proteolytic cleavage; it is also regulated by acetylation, phosphorylation, S-nitrosylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–100 of CASP3 (P42574). This region includes the N-terminal prodomain; consider that the antibody may recognize both pro‑ and cleaved forms depending on epitope accessibility. Verify specificity for active Caspase‑3 in the relevant biological system.
• For Western blot (WB), active Caspase‑3 may appear as cleaved fragments (e.g., p19, p17) rather than the full‑length 32 kDa proform. Use appropriate apoptotic inducers and positive controls (e.g., staurosporine‑treated cells) to confirm detection of the active form.
• Due to its cytoplasmic localization, prepare cytosolic extracts for optimal detection in WB or ELISA.
• The antibody is validated for WB and ELISA with human samples. For other applications or species, cross‑reactivity should be verified experimentally.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers a comprehensive one‑stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our portfolio supports apoptosis research with reliable tools for detecting active Caspase‑3, and we can assist in obtaining rare‑target reagents or custom‑developed materials to accelerate your diagnostic workflows.