Anti-ACSS3 Rabbit Polyclonal Antibody for WB and ELISA - Q9H6R3

Core Product Specifications and Parameters

Biological Background: ACSS3 Function and Localization

• ACSS3 is a mitochondrial acyl-CoA synthetase short-chain family member 3, also known as acetate--CoA ligase 3 or propionate--CoA ligase, encoded by the ACSS3 gene (UniProt Q9H6R3).
• It catalyzes the formation of acetyl-CoA from short-chain fatty acids, playing a key role in lipid metabolism. Related references: PMID:28003429
• Propionate is the preferred substrate; acetate and butyrate are utilized with lower affinity (By similarity).
• The protein localizes to the mitochondrial matrix, consistent with the presence of a transit peptide.
• Post-translational modifications include acetylation, and the protein undergoes alternative splicing (UniProt keywords).
• Predicted molecular weight is approximately 75 kDa, which may vary due to modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–280 of human ACSS3. For Western blot, consider using reducing conditions and validate the detected band near the predicted molecular weight of 75 kDa.
• For ELISA, optimize coating and detection conditions based on standard protocols, and include appropriate positive and negative controls.
• Confirm cross-reactivity with mouse and rat samples, as indicated, by comparing sequence homology if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibodies like this ACSS3 polyclonal antibody, to support your diagnostic and research development. As a one-stop sourcing partner, we provide bulk ancillary reagents, optimized antibody pairs, and access to rare target raw materials for short-chain fatty acid metabolism and mitochondrial research. Contact us to streamline your supply chain from concept to clinic.