Anti-ACSS2 Polyclonal Antibody for WB, ELISA - Q9NR19

Core Product Specifications and Parameters

Biological Background: ACSS2 Function and Localization

• ACSS2 encodes cytoplasmic acetyl-coenzyme A synthetase, a member of the acyl-CoA synthetase short-chain family, also known as AceCS1.
• Catalyzes the synthesis of acetyl-CoA from acetate (preferred substrate) and CoA, with lower affinity for propionate, and also converts lactate to lactoyl-CoA; does not act on butyrate or crotonate. Related references: PMID:10843999 PMID:28003429 PMID:28552616 PMID:38369012 PMID:39561764
• Central to lipid metabolism and energy homeostasis, linking acetate metabolism to acetyl-CoA pools for fatty acid and cholesterol synthesis.
• Under glucose deprivation, AMPK-mediated phosphorylation triggers nuclear translocation of ACSS2, where it binds TFEB and locally produces acetyl-CoA for histone acetylation, promoting lysosomal biogenesis, autophagy and tumorigenesis. Related references: PMID:28552616
• Acts as a chromatin-bound transcriptional coactivator for memory-related neuronal genes, maintaining local acetyl-CoA pools to support histone acetylation and gene expression (By similarity).
• Primarily localizes to the cytoplasm and cytosol, but translocates to the nucleus upon phosphorylation.
• Post-translationally modified by acetylation and phosphorylation; involved in lipid metabolism, transcription regulation, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 462-701), suggesting the antibody may recognize both full-length and potential C-terminal truncation variants.
• For WB, the predicted molecular weight is ~79 kDa; consider using a suitable protein ladder and positive control lysates (e.g., HeLa, HepG2).
• Given the nuclear localization under certain conditions, subcellular fractionation or nuclear extract preparation may be considered to detect nuclear ACSS2.
• For ELISA, using the recombinant immunogen as a coating antigen or standard can serve as a positive control.
• Cross-reactivity is reported for human and rat; validate in other species of interest before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw materials, offering a broad portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This ACSS2 antibody supports research in metabolic disorders, cancer metabolism, and epigenetic regulation. From concept to clinic, we provide the raw materials and technical support to streamline your assay development.