Anti-ACSS1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NUB1

Core Product Specifications and Parameters

Biological Background: Acetyl-coenzyme A synthetase 2-like, mitochondrial Function and Localization

• ACSS1 encodes acetyl-coenzyme A synthetase 2-like, mitochondrial, also known as acetate--CoA ligase 2, acetyl-CoA synthetase 2 (AceCS2), and propionate--CoA ligase.
• Catalyzes the synthesis of acetyl-CoA from acetate and CoA, with acetate as the preferred substrate; propionate is utilized with much lower affinity. Related references: PMID:16788062
• Under ketogenic conditions, provides acetyl-CoA for oxidation and contributes to thermogenesis, using acetate as an alternative fuel when carbohydrates are limited.
• Localizes to the mitochondrial matrix, where mitochondrial fatty acid metabolism occurs.
• Contains ATP-binding and nucleotide-binding motifs, typical of the acyl-CoA synthetase short-chain family.
• Undergoes acetylation and is subject to alternative splicing, indicating potential post-translational and transcriptional regulation.
• Predicted molecular weight is approximately 75 kDa (UniProt: 74857 Da).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 340-689 of human ACSS1, targeting the C-terminal region; verify reactivity against full-length protein in your system.
• For Western blot (WB), expect a band near 75 kDa; use positive controls from human, mouse, or rat cells expressing ACSS1.
• In IHC-P and IF/ICC, mitochondrial staining should be confirmed with organelle markers; optimize antigen retrieval and permeabilization as needed.
• ELISA applications may benefit from the recombinant immunogen to ensure capture/detection of native protein; validate cross-reactivity empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, offering validated research tools such as the ACSS1 polyclonal antibody for lipid metabolism and mitochondrial studies. Our supply network includes optimized antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development and diagnostic workflows.