Anti-ACSM5 Polyclonal Antibody for WB, ELISA - Q6NUN0

Core Product Specifications and Parameters

Biological Background: ACSM5 Function and Localization

• ACSM5 (Acyl-CoA synthetase medium-chain family member 5, also known as MACS3) is a mitochondrial enzyme that catalyzes the ATP-dependent activation of medium-chain fatty acids to their corresponding acyl-CoA thioesters, the first committed step in fatty acid metabolism.
• It negatively regulates lipid accumulation by inhibiting FABP4-PPARgamma signaling. Related references: PMID:37957699
• The protein is localized to the mitochondrial matrix.
• Tissue specificity: Detected in kidney and liver.
• Features a transit peptide for mitochondrial import and undergoes acetylation and alternative splicing.
• Contains ATP/GTP-binding domains and requires magnesium for catalytic activity, characteristic of the acyl-CoA synthetase ligase family.
• Part of the medium-chain acyl-CoA synthetase family; identified as a reference proteome component with proteomics confirmation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 27-130 of human ACSM5; consider performing peptide competition assays to confirm binding specificity.
• For WB, use whole-cell or tissue lysates from kidney and liver, where ACSM5 expression is detected. Optimize loading amounts and antibody dilution based on the expected 64 kDa band.
• Validate antibody performance in the relevant sample system (e.g., human cell lines or tissue homogenates) and include appropriate positive/negative controls.
• For ELISA, coat plates with the immunogen peptide or recombinant protein and titrate the antibody to determine optimal working concentration.
• Cross-reactivity has been confirmed only with human; if working with other species, verify sequence homology and conduct cross-reactivity testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner offering comprehensive sourcing—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This ACSM5 antibody supports research in fatty acid metabolism and metabolic disorders, and our team can assist with rare target sourcing and procurement to accelerate your assay development.