Anti-ACSM3 Rabbit Polyclonal Antibody for WB, ELISA - Q53FZ2

Core Product Specifications and Parameters

Biological Background: ACSM3 Function and Localization

• ACSM3 is a mitochondrial acyl-coenzyme A synthetase medium-chain family member 3, also known as butyrate--CoA ligase 3 or protein SA homolog (UniProt: Q53FZ2).
• It catalyzes the activation of fatty acids by CoA to produce acyl-CoA, the first step in fatty acid metabolism, with a preference for isobutyrate among 2-6 carbon fatty acids (PubMed:11772874). Related references: PMID:11772874
• ACSM3 is localized to the mitochondrial matrix, consistent with its role in mitochondrial fatty acid oxidation.
• The enzyme belongs to the medium-chain acyl-CoA synthetase family and is characterized by ATP-binding and ligase activity.
• Key post-translational modifications include acetylation, and the protein binds magnesium as a cofactor.
• Alternatively spliced isoforms have been reported, and the protein contains a transit peptide for mitochondrial import.
• Functional studies indicate involvement in butyrate and propionate metabolism, linking it to short/medium-chain fatty acid utilization.

Experimental Guidance and Technical Tips

• The immunogen is designed from the N-terminal region (aa 28-130), which may recognize both full-length and possibly processed isoforms. Consider verifying reactivity with endogenous protein in relevant samples.
• For Western blot, the predicted molecular weight is 66 kDa. Ensure proper mitochondrial protein extraction and use appropriate reducing conditions.
• For ELISA applications, validate the antibody with recombinant or purified ACSM3 protein to establish sensitivity.
• Cross-reactivity with mouse is expected; verify in mouse tissue lysates to confirm specificity for orthologous ACSM3.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your fatty acid metabolism research and diagnostic assay development. Our ACSM3 rabbit polyclonal antibody exemplifies our commitment to offering high-quality reagents for mitochondrial biology studies.