Anti-ACSM2A Polyclonal Antibody for WB, ELISA - Q08AH3

Core Product Specifications and Parameters

Biological Background: ACSM2A Function and Localization

• ACSM2A (Acyl-coenzyme A synthetase ACSM2A, mitochondrial) is a member of the acyl-CoA synthetase medium-chain family, also known as middle-chain acyl-CoA synthetase 2A.
• It catalyzes the ATP-dependent activation of medium-chain fatty acids (C4–C10) to acyl-CoA thioesters, the first step in fatty acid β-oxidation and lipid metabolism.
• The enzyme also ligates CoA to carboxylate-containing xenobiotics such as benzoate, implicating it in detoxification pathways.
• ACSM2A localizes to the mitochondrion, consistent with its role in mitochondrial fatty acid metabolism.
• Key structural features include ATP-binding, magnesium-binding, and a nucleotide-binding domain, characteristic of the acyl-CoA synthetase family.
• Post-translational modifications include phosphorylation, as indicated by proteomics identification; the protein contains a transit peptide for mitochondrial import.
• The enzyme exhibits butyrate--CoA ligase activity, playing a role in butyrate metabolism, an important short-chain fatty acid.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 418–577), which may yield antibodies recognizing both native and denatured protein; consider this for Western blot under reducing conditions.
• For ELISA, the recombinant protein or peptide antigen may serve to verify reactivity; validate in the relevant species (human, mouse, rat) with appropriate sample systems.
• Given mitochondrial localization, cell fractionation or mitochondrial enrichment could enhance detection sensitivity in Western blot assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research labs with a comprehensive portfolio of validated IVD raw materials, including this anti-ACSM2A rabbit polyclonal antibody. We offer optimized polyclonal antibodies, antibody pairs, and bulk ancillary reagents for metabolic and mitochondrial target assays, coupled with expert technical consultation to streamline your development from concept to clinic.