Anti-ACSL6 Polyclonal Antibody for WB, ELISA - Q9UKU0

Core Product Specifications and Parameters

Biological Background: ACSL6 Function and Localization

• ACSL6 (also known as ACS2, FACL6, LACS5, LACS6) is a long-chain-fatty-acid--CoA ligase that converts long-chain fatty acids to acyl-CoA, a crucial step for both lipid synthesis and degradation via β-oxidation. Related references: PMID:22633490, PMID:24269233
• The enzyme plays a key role in brain fatty acid metabolism; the acyl-CoA products it generates are utilized exclusively for the synthesis of brain lipids.
• ACSL6 localizes to the membranes of mitochondria, peroxisomes, microsomes, and the endoplasmic reticulum, reflecting its involvement in distinct organelle-specific lipid metabolic pathways.
• Tissue specificity: ACSL6 is expressed predominantly in erythrocyte precursors, including reticulocytes and fetal blood cells, as well as in hematopoietic stem cells from cord blood, bone marrow, and brain.
• The protein belongs to the long-chain acyl-CoA synthetase family and binds ATP and magnesium, consistent with its ligase activity.
• Keywords include alternative splicing and chromosomal rearrangement, indicating potential isoform diversity and genetic variation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 383–622 of human ACSL6, a region that may be conserved across species; cross-reactivity with mouse and rat supports this.
• For Western blot, a band around 78 kDa is expected; consider optimizing gel percentage and transfer conditions to resolve the protein.
• ELISA can be employed for detecting native or recombinant ACSL6; validate the assay in relevant sample systems such as brain or erythrocyte lysates.
• Pre-adsorption or blocking with the immunogen may be considered to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing of rare targets to support your diagnostic and research needs. This ACSL6 polyclonal antibody supports studies in fatty acid metabolism and lipid biology, and CamelBio can assist with procurement of complementary reagents and technical consulting to accelerate your project from concept to clinic.