Anti-ACSL5 Rabbit Polyclonal Antibody for WB, ELISA - Q9ULC5

Core Product Specifications and Parameters

Biological Background: ACSL5 Function and Localization

• ACSL5, also known as arachidonate--CoA ligase or long-chain acyl-CoA synthetase 5 (LACS 5), is encoded by the ACSL5 gene (aliases ACS5, FACL5). It catalyzes the conversion of long-chain fatty acids to their active CoA esters, which are essential for lipid synthesis and beta-oxidation. Related references: PMID:17681178, PMID:22633490, PMID:24269233
• ACSL5 preferentially utilizes C16-C18 unsaturated fatty acids and may activate exogenous fatty acids for triacylglycerol storage, contributing to intracellular lipid accumulation.
• The enzyme localizes to multiple compartments, including mitochondria, endoplasmic reticulum, and the cell membrane, reflecting its roles in lipid metabolism and signaling.
• At the villus tip of the small intestine, ACSL5 sensitizes epithelial cells to TRAIL-induced apoptosis, and it may also support glioma cell survival.
• Post-translational modifications include acetylation and phosphorylation, and the protein undergoes alternative splicing.
• Key functional keywords associated with ACSL5 include ATP-binding, fatty acid metabolism, lipid metabolism, magnesium, and membrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 500-739) of human ACSL5, which may contain conserved epitopes; verify cross-reactivity in your specific species or model system.
• For Western blotting, the expected band is approximately 76 kDa (consistent with UniProt data). Optimize gel percentage and transfer conditions, and include appropriate positive and negative controls.
• In ELISA applications, polyclonal antibodies can offer enhanced sensitivity due to epitope diversity, but coating and detection conditions should be empirically optimized.
• Cross-reactivity with human, mouse, and rat makes this antibody potentially useful for translational studies in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This anti-ACSL5 antibody supports investigations into fatty acid metabolism and lipid-related disorders, and we invite you to leverage our integrated procurement solutions to accelerate your development from concept to clinic.