Anti-ACSL5 Polyclonal Antibody for WB and ELISA - Q9ULC5

Core Product Specifications and Parameters

Biological Background: ACSL5 Function and Localization

• ACSL5 (encoded by the ACSL5 gene) is a long-chain-fatty-acid--CoA ligase 5, also known as arachidonate--CoA ligase or LACS 5.
• Catalyzes the conversion of long-chain fatty acids into their active CoA derivatives for incorporation into cellular lipids or degradation via beta-oxidation. Related references: PMID:17681178 PMID:22633490 PMID:24269233
• Activates exogenous fatty acids for triacylglycerol synthesis and intracellular storage.
• Preferentially utilizes a wide range of saturated fatty acids, with a higher preference for C16-C18 unsaturated fatty acids.
• Localizes to the mitochondrion, endoplasmic reticulum, and cell membrane; specifically associates with the mitochondrial outer membrane and ER membrane.
• At the villus tip of the small intestine, ACSL5 may sensitize epithelial cells to TRAIL-induced apoptosis.
• May play a role in glioma cell survival, suggesting relevance in cancer biology.
• Post-translational modifications include acetylation and phosphorylation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 500–739) of human ACSL5; verify recognition if your target includes N-terminal modifications or variants.
• For Western blot, anticipate a band at ~76 kDa; consider running a positive control (e.g., ACSL5-transfected cell lysate) for validation.
• The antibody cross-reacts with human, mouse, and rat; confirm performance in your specific sample type and species.
• For ELISA, the recombinant immunogen protein can serve as a positive control or coating antigen during assay optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical services, bridging the gap from target discovery to clinical application. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support research on fatty acid metabolism and lipid signaling. Partner with us for customized sourcing solutions and accelerate your diagnostic development with reliable, high-quality materials.