Anti-ACSL4 Rabbit Polyclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - O60488

Core Product Specifications and Parameters

Biological Background: ACSL4 Function and Localization

• ACSL4 (also known as FACL4, LACS4, ACS4) is a member of the long-chain acyl-CoA synthetase family that converts free long-chain fatty acids into acyl-CoA esters.
• Catalyzes the formation of acyl-CoA from long-chain fatty acids, a critical step for both lipid synthesis and β-oxidation; preferentially activates arachidonate (20:4n-6) and eicosapentaenoate (20:5n-3). Related references: PMID:21242590, PMID:22633490, PMID:24269233
• Modulates glucose-stimulated insulin secretion by regulating levels of unesterified epoxyeicosatrienoic acids (EETs).
• Regulates prostaglandin E2 secretion, linking fatty acid activation to inflammatory signaling. Related references: PMID:21242590
• Acts as a critical activator of ferroptosis by converting polyunsaturated fatty acids (especially arachidonate and adrenate) into their acyl-CoA forms, which serve as primary substrates for lipid peroxidation. Related references: PMID:27842070, PMID:35027735, PMID:38720107
• Localizes to the mitochondrial outer membrane, endoplasmic reticulum membrane, and cell membrane, reflecting its roles in different subcellular lipid compartments.
• Post-translational modifications include phosphorylation; the enzyme requires ATP and magnesium for activity and contains a transmembrane helix for membrane anchoring.
• Disease associations: mutations in ACSL4 are linked to Alport syndrome, sensorineural deafness, elliptocytosis, hereditary hemolytic anemia, and X-linked intellectual disability (XLID).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 42–321 of human ACSL4 (NP_075266.1); this region is expected to generate antibodies that recognize endogenous full-length ACSL4.
• For western blot, the predicted band weight is approximately 79 kDa. Validate specificity using appropriate positive and negative controls (e.g., siRNA knockdown, knockout lysates, or over-expression).
• This antibody has been reported in WB, IP, IF/ICC, IHC-P, and ELISA. Optimize dilution factors for each application; typical starting dilutions for rabbit polyclonal antibodies range from 1:500–1:2000 for WB and 1:50–1:500 for IHC-P, but empirical testing is essential.
• Cross-reactivity is expected with human, mouse, and rat ACSL4. Sequence alignment of the immunogen region across species may guide potential reactivity with other organisms.
• For functional studies targeting ferroptosis, consider co-treatment with ferroptosis inducers (e.g., erastin, RSL3) or inhibitors (e.g., ferrostatin-1, liproxstatin-1) to assess ACSL4-mediated lipid peroxidation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-ACSL4 polyclonal antibody as part of a comprehensive IVD raw-material portfolio for diagnostic and research applications. Beyond this antibody, we provide validated antibody pairs, bulk ancillary reagents, and custom sourcing for other fatty acid metabolism and ferroptosis-related targets. With one-stop procurement and dedicated technical support, CamelBio helps streamline your assay development from concept to clinic.