Anti-ACSL4 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - O60488

Core Product Specifications and Parameters

Biological Background: ACSL4 Function and Localization

• ACSL4 (long-chain-fatty-acid--CoA ligase 4), also known as ACS4, FACL4, or LACS4, is a key enzyme that activates long-chain fatty acids by converting them to acyl-CoA derivatives, which are required for both cellular lipid synthesis and beta-oxidation. Related references: PMID:21242590 PMID:22633490 PMID:24269233
• It preferentially utilizes arachidonate and eicosapentaenoate as substrates, and also activates various epoxyeicosatrienoic acid (EET) regioisomers, with highest activity toward 8,9-EET. Related references: PMID:21242590
• ACSL4 plays a critical role in ferroptosis by activating polyunsaturated fatty acids, particularly arachidonate and adrenate, to generate the primary lipid peroxidation substrates that drive this form of regulated cell death. Related references: PMID:27842070 PMID:35027735 PMID:38720107
• It modulates prostaglandin E2 secretion, contributing to lipid mediator signaling. Related references: PMID:21242590
• The enzyme localizes to the mitochondrial outer membrane, endoplasmic reticulum membrane, and cell membrane, reflecting its involvement in multiple subcellular lipid metabolic compartments.
• ACSL4 is associated with various diseases based on UniProt keywords, including Alport syndrome, deafness, X-linked intellectual disability, hereditary hemolytic anemia, and elliptocytosis, highlighting its broad physiological importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 42-321 of human ACSL4, a region that may contain conserved epitopes across species; the reported cross-reactivity with mouse and rat supports this, but always validate in your experimental system.
• For Western blotting, expect a band at approximately 79 kDa. Consider optimizing antibody dilution and using appropriate loading controls to confirm specificity.
• In immunofluorescence/ICC, anticipate staining at mitochondria, ER, and plasma membrane; co-localization studies with organelle markers are recommended to verify subcellular distribution.
• For immunoprecipitation and ELISA, the antibody may be suitable, but each application requires individual optimization of antibody concentration and buffer conditions.
• Given the involvement of ACSL4 in ferroptosis and fatty acid metabolism, this antibody can be a valuable tool for studies in cancer biology, metabolic disorders, and lipid signaling; however, all experimental conditions should be validated in the researcher's model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by providing a one-stop platform for diagnostic manufacturers, labs, and research institutes. This anti-ACSL4 rabbit monoclonal antibody, targeting fatty acid metabolism and ferroptosis pathways, is part of our extensive catalog of validated antibodies suitable for multiple applications. In addition, we offer monoclonal/polyclonal antibody development, antibody pairs, bulk ancillary reagents, and can source rare targets, supporting every stage from concept to clinical development.