Anti-ACSL1 Rabbit PolymAb® Monoclonal Antibody for WB, IHC, ELISA - P33121

Core Product Specifications and Parameters

Biological Background: ACSL1 Function and Localization

• ACSL1 catalyzes the conversion of long-chain fatty acids to acyl-CoAs, crucial for lipid synthesis and beta-oxidation. Related references: PMID:21242590, PMID:22633490, PMID:24269233
• It preferentially utilizes palmitoleate, oleate, and linoleate as substrates. Related references: PMID:24269233
• ACSL1 exhibits higher activity with arachidonate compared to epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).
• The protein localizes to the mitochondrial outer membrane, peroxisome membrane, microsome membrane, and endoplasmic reticulum membrane, reflecting its role in multiple organelles.
• ACSL1 is highly expressed in liver, heart, skeletal muscle, kidney, and erythroid cells, with moderate expression in brain, lung, placenta, and pancreas.
• Post-translational modifications include acetylation, nitration, glycosylation, and phosphorylation, as indicated by proteomics studies.
• The gene undergoes alternative splicing, yielding multiple transcript variants.
• ACSL1 is also known by aliases such as ACS1, LACS, FACL1, FACL2, LACS1, and LACS2.

Experimental Guidance and Technical Tips

• For Western blotting, a band near 78 kDa is expected; include appropriate loading controls and validate in the relevant sample system.
• In immunohistochemistry, consider using liver or heart tissue as positive controls due to high ACSL1 expression.
• The immunogen targets the N-terminal 1-100 amino acids; verify recognition of endogenous protein and potential isoforms in your experimental model.
• Cross-reactivity with mouse and rat facilitates translational studies; confirm performance in the specific strain or cell line used.
• For ELISA applications, optimize coating conditions and detection ranges according to your assay requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as an integrated partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This ACSL1 antibody supports metabolic and lipid research applications, aligning with our commitment to provide comprehensive supply solutions from concept to clinic. Our one-stop platform streamlines access to high-quality raw materials, technical services, and consulting for diagnostic manufacturers and research laboratories.