Anti-ACSL1 Rabbit Polyclonal Antibody for WB, ELISA - P33121

Core Product Specifications and Parameters

Biological Background: ACSL1 Function and Localization

• ACSL1, also known as long-chain-fatty-acid--CoA ligase 1, is a key enzyme in fatty acid metabolism, converting free long-chain fatty acids into their acyl-CoA derivatives for downstream lipid synthetic and degradative pathways. Related references: PMID:21242590 PMID:22633490 PMID:24269233
• The enzyme preferentially activates palmitoleate, oleate, and linoleate among long-chain fatty acids. Related references: PMID:24269233
• ACSL1 preferentially converts arachidonate rather than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).
• Localizes primarily to the mitochondrial outer membrane, peroxisomal membrane, microsomal membrane, and endoplasmic reticulum membrane, consistent with its roles in both anabolism and catabolism of lipids.
• Highly expressed in liver, heart, skeletal muscle, kidney, and erythroid cells, with lower expression in brain, lung, placenta, and pancreas.
• The ACSL1 gene undergoes alternative splicing, and the protein is subject to multiple post-translational modifications including acetylation, nitration, phosphorylation, and glycosylation.
• Contains key functional motifs: an ATP-binding domain, a nucleotide-binding domain, and a transmembrane helix that anchors it to membranes.

Experimental Guidance and Technical Tips

• For Western blotting, optimize antibody dilution in the relevant sample system; the full-length protein migrates at approximately 76–77 kDa, and additional bands corresponding to isoforms or degradation products may be observed due to the polyclonal nature.
• In ELISA applications, the peptide immunogen can be employed as a standard for quantitative assays; consider pairing this antibody with a detection antibody recognizing a distinct epitope.
• Cross-reactivity with mouse ACSL1 has been documented, making this antibody suitable for studies in mouse models; validate in the specific tissue or cell line of interest.
• Given the multiple subcellular localizations of ACSL1, fractionation controls or organelle markers can help confirm specificity in localization studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic developers and research laboratories. This anti-ACSL1 polyclonal antibody supports investigations into fatty acid metabolism, lipid disorders, and related disease mechanisms. As a trusted partner, CamelBio also offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to advance your assay development.