Anti-ACSL1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P33121

Core Product Specifications and Parameters

Biological Background: ACSL1 Function and Localization

• ACSL1 (Long-chain-fatty-acid–CoA ligase 1) is a key metabolic enzyme that catalyzes the ATP-dependent formation of fatty acyl-CoA thioesters from free long-chain fatty acids, enabling their utilization in lipid synthesis and mitochondrial β-oxidation. Related references: PMID:21242590, PMID:22633490, PMID:24269233
• It preferentially utilizes unsaturated fatty acids such as palmitoleate, oleate, and linoleate, and also activates arachidonate to arachidonoyl-CoA, linking it to eicosanoid biosynthesis.
• ACSL1 localizes to the mitochondrial outer membrane, peroxisomal membrane, endoplasmic reticulum, and microsomes, reflecting its dual role in energy metabolism and complex lipid assembly.
• The protein is most highly expressed in tissues with heavy fatty acid flux, including liver, heart, skeletal muscle, kidney, and erythroid cells; lower expression occurs in brain, lung, placenta, and pancreas.
• Post-translational modifications include acetylation, nitration, and phosphorylation, and it is classified as a glycoprotein; alternative splicing produces multiple isoforms.
• It is a single-pass membrane protein with a signal-anchor domain, anchoring it to intracellular membranes.

Experimental Guidance and Technical Tips

• For WB, the predicted molecular weight is approximately 78 kDa; verify against your sample lysate and include positive controls from high-expression tissues such as liver or heart.
• In immunofluorescence, the enzyme’s multiple organelle membrane localizations can produce punctate or reticular staining; co-staining with organelle-specific markers is recommended for confirmation.
• The immunogen resides within the C-terminal region (aa 598–698), so the antibody may recognize all known isoforms if the epitope is conserved; validate specificity by peptide competition or knockdown if needed.
• Cross-reactivity with mouse and rat is predicted; however, check sequence alignment for the immunogen region in your species of interest to avoid false negatives.
• ELISA and IHC-P are listed applications; for IHC, antigen retrieval and antibody dilution will require optimization in the target tissue.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material supplier, CamelBio provides this ACSL1 monoclonal antibody alongside a wide range of validated reagents for lipid metabolism research and diagnostic assay development. Our catalog includes optimized antibody pairs, bulk ancillary reagents, and sourcing services for rare and custom targets, ensuring a seamless bridge from early R&D to clinical application. Contact CamelBio to discuss your specific project needs and streamline your supply chain.