Anti-ACSL1 Polyclonal Antibody for WB, IP, IF/ICC - P33121

Core Product Specifications and Parameters

Biological Background: ACSL1 Function and Localization

• ACSL1 encodes a long-chain-fatty-acid--CoA ligase that converts fatty acids to acyl-CoAs, supporting both lipid synthesis and beta-oxidation. It preferentially uses palmitoleate, oleate, and linoleate.
  Related references: PMID:21242590, PMID:22633490, PMID:24269233
• It also activates arachidonate, showing higher preference for arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs).
• The protein localizes to multiple membrane compartments, including mitochondrial outer membrane, peroxisome, microsome, and endoplasmic reticulum.
• ACSL1 is highly expressed in liver, heart, skeletal muscle, kidney, and erythroid cells, with lower levels in brain, lung, placenta, and pancreas.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• Post-translational modifications include acetylation, nitration, phosphorylation, and glycosylation, which may regulate its activity and localization.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 46-145) corresponds to a region conserved across human, mouse, and rat, supporting cross-reactivity observed.
• For Western blot, consider testing lysates from ACSL1-rich tissues such as liver or heart, and optimize antibody concentration and detection conditions.
• In immunofluorescence, use appropriate permeabilization and blocking steps given the multi-membrane localization of ACSL1.
• For immunoprecipitation, the antibody may capture native ACSL1 from whole-cell lysates; validate with appropriate positive controls.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio provides diagnostic manufacturers and researchers with high-quality antibodies like this anti-ACSL1 polyclonal antibody, along with optimized antibody pairs and bulk ancillary reagents for fatty acid metabolism research. Our comprehensive supply network supports everything from validated monoclonal/polyclonal antibodies to rare target raw materials, streamlining your assay development from concept to clinic.