Anti-ACSF3 Polyclonal Antibody for WB, IHC-P, ELISA - Q4G176

Core Product Specifications and Parameters

Biological Background: ACSF3 Function and Localization

• ACSF3 (acyl-CoA synthetase family member 3) is a mitochondrial enzyme also known as malonate--CoA ligase.
• It catalyzes the activation of malonate and methylmalonate to their CoA thioesters, initiating intramitochondrial fatty acid synthesis. Related references: PMID:21841779 / PMID:21846720
• The enzyme may exhibit a preference for very-long-chain fatty acid substrates. Related references: PMID:17762044
• ACSF3 is localized to the mitochondrion, consistent with its role in mitochondrial fatty acid and lipid metabolism.
• The protein contains ATP-binding and nucleotide-binding domains, characteristic of the acyl-CoA synthetase/ligase family.
• UniProt keywords include ATP-binding, Fatty acid metabolism, Ligase, Lipid metabolism, Mitochondrion, Nucleotide-binding, Proteomics identification, Reference proteome, and Transit peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 370-576 of human ACSF3, which covers a large portion of the C-terminal region; the resulting polyclonal antibody may recognize multiple epitopes across this region.
• For western blotting, the predicted molecular weight of ACSF3 is approximately 64 kDa; a specific band may be observed near this size, but validation in the relevant sample system is recommended.
• This antibody has been tested in WB, IHC-P, and ELISA; consider optimizing dilution and detection conditions for each application.
• Cross-reactivity with mouse ACSF3 extends the utility of this antibody to mouse model studies; however, sequence homology should be checked for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to IVD raw materials and technical support—from early-stage development to clinical application. This anti-ACSF3 polyclonal antibody supports research into mitochondrial fatty acid metabolism and related disorders, and we can also source validated antibody pairs, bulk ancillary reagents, and difficult-to-find raw materials for your assay development pipeline. Please contact us to discuss your specific procurement needs.