Anti-ACRV1 Polyclonal Antibody for WB, ELISA - P26436

Core Product Specifications and Parameters

Biological Background: ACRV1 (SP-10) Function and Localization

• ACRV1 (Acrosomal Vesicle Protein 1), also known as SP-10 (Sperm Protein 10) or SPACA2, is encoded by the ACRV1 gene located on chromosome 11q13 in humans.
• The protein is classified as an acrosomal component, specifically localized to the acrosome, a cap-like secretory vesicle covering the anterior portion of the sperm nucleus.
• ACRV1 expression is highly testis-specific, consistent with its role in sperm development and acrosome biogenesis.
• According to UniProt, ACRV1 contains multiple repeat regions and a signal peptide, suggesting it is a secreted glycoprotein that undergoes post-translational processing.
• Post-translational modifications include glycosylation, and the protein is subject to alternative splicing, which may generate distinct isoforms.
• The mature protein has a molecular weight of approximately 28 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 22–265, representing a large portion of the extracellular region of human ACRV1. This design aims to recognize both full-length and processed forms of the protein.
• For Western blot analysis, a band near 28 kDa is expected in human testis tissue lysates. Given cross-reactivity with rat, rodent models may be suitable for validation studies.
• Since ACRV1 is a glycoprotein, enzymatic deglycosylation can be employed to verify band specificity and investigate glycoforms.
• The antibody is applicable in ELISA; consider using it as a capture antibody paired with a detection antibody, or as a detection antibody in indirect ELISA setups, with appropriate optimization of coating and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering a complete range of products from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents. In the field of reproductive biology and infertility diagnostics, the ACRV1 target is particularly relevant for assessing sperm function and acrosome integrity. Our supply network is designed to support diagnostic manufacturers with consistent quality and scalable procurement for both research and commercial assay development.