Anti-ACP5 Polyclonal Antibody for WB, IF, IHC, ELISA - P13686

Core Product Specifications and Parameters

Biological Background: ACP5 Function and Localization

• ACP5 encodes tartrate-resistant acid phosphatase type 5 (TRAP, also known as TrATPase), a member of the acid phosphatase family responsible for dephosphorylation reactions.
• The enzyme plays a key role in bone metabolism by dephosphorylating osteopontin and bone sialoprotein, crucial for osteoclast function and bone resorption.
• ACP5 is a lysosomal enzyme; it is synthesized with a signal peptide that directs it to the lysosome, where it exerts its catalytic activity.
• It requires iron as a cofactor, making it a metalloenzyme, and contains disulfide bonds and glycosylation sites important for its structure and stability.
• Elevated ACP5 expression is observed in pathological conditions including Gaucher disease, Hodgkin disease, and various leukemias (hairy cell, B-cell, and T-cell), suggesting its potential as a biomarker.
• The protein has been characterized through direct protein sequencing and 3D structure determination, confirming its identity and providing insights into its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 76-325 of human ACP5, which includes the catalytic domain; thus, the antibody likely detects the mature enzyme form. Consider validating in samples expressing active osteoclasts or leukemic cell lines.
• For Western blot, the predicted molecular weight is 37 kDa; however, glycosylation may cause a shift. Optimize sample preparation and use appropriate controls.
• For immunohistochemistry (paraffin-embedded, IHC-P), antigen retrieval may be necessary; test with both enzymatic and heat-induced methods.
• In immunofluorescence, permeabilization is required to detect the lysosomal localization; co-staining with lysosomal markers can confirm specificity.
• Cross-reactivity with mouse and rat allows for translational studies; validate in your specific model system.
• ELISA performance should be verified with recombinant protein or biological samples; consider pairing with a detection antibody if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our ACP5 antibody supports bone metabolism and oncology research, with cross-species reactivity enabling diverse experimental applications. For rare target raw-material sourcing and technical consultation from concept to clinic, CamelBio serves as your reliable one-stop partner.