Anti-ACP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P24666

Core Product Specifications and Parameters

Biological Background: ACP1 Function and Localization

• ACP1, also known as low molecular weight phosphotyrosine protein phosphatase (LMW-PTP), is a cytoplasmic enzyme with an approximate molecular weight of 18 kDa.
• It catalyzes the dephosphorylation of tyrosine-phosphorylated proteins, low-molecular-weight aryl phosphates, and acyl phosphates, with substrate specificity differing between its two major isoforms.
• ACP1 is expressed in T-lymphocytes, suggesting a role in immune cell signaling pathways.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may regulate its activity.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• Keywords from UniProt: 3D-structure, acetylation, alternative splicing, cytoplasm, direct protein sequencing, hydrolase, phosphoprotein, protein phosphatase, proteomics identification, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full-length human ACP1 sequence (amino acids 1–158). For Western blot, expect a band near 18 kDa; ensure proper resolution by using 12–15% SDS-PAGE gels.
• This antibody is validated for WB, IHC-P, and ELISA. For IHC-P, consider optimizing antigen retrieval and testing on lymphocyte-rich tissues (e.g., tonsil) as a positive control.
• The polyclonal nature may introduce batch variability; it is advisable to validate each new lot in the intended assay system.
• Cross-reactivity with mouse ACP1 is indicated, making it suitable for rodent model studies; confirm sequence homology if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For researchers studying phosphotyrosine signaling and acid phosphatase biology, CamelBio streamlines procurement of rare-target reagents, including anti-ACP1 antibodies, to accelerate diagnostic development from concept to clinic. Our one-stop supply chain ensures consistent quality and scalability for your assay needs.