Anti-ACOX2 Polyclonal Antibody for WB, IHC-P, ELISA - Q99424

Core Product Specifications and Parameters

Biological Background: ACOX2 Function and Localization

• ACOX2 (peroxisomal acyl-coenzyme A oxidase 2) is also known as BCOX, BRCOX, CBAS6, THCCox, or BRCACOX.
• ACOX2 catalyzes the initial and rate-limiting dehydrogenation step in peroxisomal beta-oxidation of branched-chain fatty acids and bile acid intermediates, producing hydrogen peroxide as a by-product. Related references: PMID:29287774
• It stereospecifically oxidizes substrates with (2S)-methyl groups, such as (2S)-pristanoyl-CoA, and exhibits redundancy with ACOX3. Related references: PMID:29287774
• In the liver, ACOX2 mediates side-chain beta-oxidation of C27 bile acyl-CoA intermediates with a (25S)-methyl group, a critical step in peroxisomal bile acid synthesis. Related references: PMID:27884763 PMID:29287774
• In brown adipose tissue, ACOX2 participates in thermogenesis by beta-oxidizing monomethyl branched-chain fatty acids, coupling to AMPK activation and mitochondrial ATP production.
• It can also oxidize straight-chain fatty acyl-CoAs (e.g., C10-CoA, C16-CoA) with low efficiency. Related references: PMID:29287774
• Subcellular localization: peroxisome.
• Tissue specificity: expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, with highest levels in heart, liver, and kidney.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-150) of human ACOX2; this polyclonal antibody may recognize multiple isoforms, suitable for total ACOX2 detection.
• For Western blotting, expect a band at approximately 77 kDa. Optimize sample preparation (e.g., reducing conditions) and antibody dilution for your specific experimental setup.
• For IHC-P, use positive control tissues like liver or kidney, and consider antigen retrieval methods.
• The antibody cross-reacts with human and mouse ACOX2; verify cross-reactivity in other species if needed.
• Use an anti-rabbit secondary antibody for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents from concept to clinic. This anti-ACOX2 polyclonal antibody is ideal for research into peroxisomal disorders, lipid metabolism, and bile acid synthesis. For bulk orders, custom sourcing, or technical consultation, contact CamelBio to streamline your diagnostic development.