Anti-ACOX1 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q15067

Core Product Specifications and Parameters

Biological Background: ACOX1 Function and Localization

• ACOX1 catalyzes the initial and rate-limiting step in the peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids (VLCFAs).
  Related references:
  PMID:15060085
  PMID:17458872
  PMID:17603022

• The enzyme desaturates 2,3-saturated acyl-CoA to (2E)-enoyl-CoA, reducing molecular oxygen to hydrogen peroxide (H₂O₂).
  Related references:
  PMID:17458872
  PMID:17603022
  PMID:7876265

• It shows highest in vitro activity toward medium-chain substrates, particularly decanoyl-CoA (C10), yet is also capable of processing long-chain and very-long-chain fatty acyl-CoAs.

• ACOX1 may participate in polyunsaturated fatty acid (PUFA) biosynthesis by regulating systemic docosahexaenoic acid (DHA) levels through peroxisomal beta-oxidation of tetracosahexaenoic acid (THA).

• The protein localizes exclusively to the peroxisome, functioning as a flavoprotein oxidase that requires FAD as a cofactor.

• Expression is widespread: isoform 1 predominates in liver and kidney, while isoform 2 is higher in brain, lung, muscle, white adipose tissue, and testis; both isoforms are nearly equal in heart.

• Post-translational modifications include acetylation and phosphorylation; pathogenic variants in ACOX1 are associated with deafness and neuropathy.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (aa 1–270); consider that the antibody may recognize both major isoforms if this domain is conserved, but validation against specific isoforms is advised.
• For Western blot (WB), the predicted molecular weight is ~74 kDa; include appropriate positive controls (e.g., human, mouse, or rat peroxisome-rich tissue lysates).
• Immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P) should incorporate permeabilization steps to allow access to the peroxisomal lumen. Counterstaining with a peroxisomal marker can help confirm localization.
• ELISA applications may utilize the antibody for capture or detection; titrate and validate in the relevant sample matrix.
• Cross-reactivity with mouse and rat enables translational studies in rodent models; verify signal specificity with gene knockout or knockdown controls where possible.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated provider of IVD raw materials, CamelBio supports diagnostic manufacturers and researchers with validated antibodies, bulk reagents, and tailored sourcing solutions for peroxisomal and metabolic disease research. This anti-ACOX1 monoclonal antibody is part of our curated portfolio for fatty acid metabolism targets, and we can also assist with custom antibody development, matched pair reagents, and ancillary consumables to streamline your assay development pipeline.