Anti-ACOX1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15067

Core Product Specifications and Parameters

Biological Background: ACOX1 Function and Localization

• ACOX1 (peroxisomal acyl-coenzyme A oxidase 1), also known as palmitoyl-CoA oxidase or straight-chain acyl-CoA oxidase (SCOX), catalyzes the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids (VLCFAs). Related references: PMID:15060085 PMID:17458872 PMID:17603022 PMID:7876265

• The enzyme desaturates 2,3-saturated acyl-CoA to 2-trans-enoyl-CoA, donating electrons directly to molecular oxygen to produce hydrogen peroxide. Related references: PMID:17458872 PMID:17603022 PMID:7876265

• Exhibits broad substrate activity, with highest activity toward medium-chain fatty acyl-CoAs (optimum at C10-decanoyl-CoA) and activity extending to long-chain and VLCFA-CoAs.

• Localized exclusively to the peroxisome, where it participates in lipid metabolism.

• Exists as alternatively spliced isoforms (isoform 1 and isoform 2) with tissue-specific expression; isoform 1 predominates in liver and kidney, while isoform 2 is higher in brain, lung, muscle, white adipose tissue, and testis, with highest overall expression in testis.

• Contains FAD as a cofactor and is subject to post-translational modifications including acetylation and phosphorylation, reflecting its regulated role in fatty acid oxidation.

• May contribute to polyunsaturated fatty acid (PUFA) biosynthesis and systemic docosahexaenoic acid (DHA) homeostasis through peroxisomal beta-oxidation of tetracosahexaenoic acid (THA), with implications in inter-organ VLCFA signaling and adipose tissue thermogenesis regulation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1–270 of human ACOX1; the antibody is expected to recognize the N-terminal region and may detect full-length and relevant isoform forms.
• For Western blot (WB), the predicted molecular weight is approximately 74 kDa; validate band specificity using appropriate controls and blocking conditions.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval protocols and include tissue-specific positive and negative controls, especially in testis, liver, and adipose tissue.
• For ELISA applications, optimize antibody coating concentration and detection reagents according to the specific assay format.
• Due to cross-reactivity with human, mouse, and rat, the antibody can be used in comparative studies across these species; verify performance in the target sample matrix to ensure consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with streamlined access to high-quality IVD raw materials. For studies involving ACOX1—a key enzyme in peroxisomal fatty acid oxidation and metabolic disorders—we offer validated monoclonal antibodies, optimized antibody pairs, and a broad portfolio of ancillary reagents suitable for assay development. Leverage our expertise in bulk supply and custom rare-target sourcing to advance your projects from concept to clinic, efficiently and reliably.