Anti-ACOT8 Rabbit Polyclonal Antibody for WB, ELISA - O14734

Core Product Specifications and Parameters

Biological Background: ACOT8 Function and Localization

• ACOT8 (Acyl-CoA Thioesterase 8) is also known as Peroxisomal acyl-CoA thioesterase 1/2 (PTE-1/PTE-2), hACTE-III, hTE, and HIV-Nef-associated acyl-CoA thioesterase, reflecting its diverse functional roles.
• Catalyzes the hydrolysis of medium- to long-chain fatty acyl-CoAs (C2-C20) into free fatty acids and coenzyme A, regulating intracellular acyl-CoA levels and peroxisomal lipid metabolism. Related references: PMID:15194431, PMID:9153233, PMID:9299485
• Also hydrolyzes bile acid-CoAs (e.g., choloyl-CoA, chenodeoxycholoyl-CoA), competing with bile acid-CoA:amino acid N-acyltransferase (BAAT) and linking ACOT8 to bile acid metabolism.
• Involved in the metabolic regulation of peroxisome proliferation, connecting ACOT8 to peroxisome biogenesis and lipid homeostasis. Related references: PMID:15194431
• May mediate HIV-Nef-induced down-regulation of CD4 cell-surface expression, highlighting a role in host-virus interactions. Related references: PMID:9153233
• Subcellularly localized to the peroxisome matrix, consistent with its functions in fatty acid oxidation and bile acid processing.
• Detected in T-cell lines and ubiquitously expressed across tissues, indicating broad physiological relevance. Related references: PMID:9153233, PMID:9299485

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1‑220) of human ACOT8; thus, the antibody is expected to recognize the full-length protein.
• For Western blotting, a band at approximately 36 kDa is anticipated in human cell or tissue lysates; consider including appropriate positive and negative controls.
• In ELISA applications, the antibody can be used as a capture or detection reagent, but coating antigen and detection conditions should be optimized.
• As a polyclonal antibody, it may exhibit reactivity against additional epitopes; validate specificity in the relevant sample system and consider pre-adsorption if needed.
• Cross-reactivity is confirmed for human samples only; performance in other species should be experimentally verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your IVD development by providing a comprehensive portfolio of raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, antigens, and bulk ancillary reagents. For ACOT8-related research in peroxisomal disorders, lipid metabolism, or viral interaction studies, we offer one-stop sourcing solutions—from concept to clinic. Let our supply chain expertise complement your diagnostic innovation with reliable, high-quality materials.