Anti-ACOT2 Polyclonal Antibody for WB, IHC-P, ELISA - P49753

Core Product Specifications and Parameters

Biological Background: ACOT2 Function and Localization

• ACOT2 (Acyl-CoA thioesterase 2) catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A, thereby regulating their intracellular levels. Related references: PMID:10944470 PMID:16940157
• It exhibits higher activity toward long-chain acyl-CoAs (C14-C20). Related references: PMID:10944470 PMID:16940157
• The enzyme is implicated in enhancing hepatic fatty acid oxidation within mitochondria.
• ACOT2 localizes predominantly to the mitochondrion.
• Strong expression is observed in heart, liver, muscle, and kidney, with weaker expression in placenta and pancreas.
• The protein is also designated as mitochondrial acyl-CoA thioesterase 2, CTE-Ia, MTE1, PTE2, PTE2A, CTE-IA, and ZAP128.
• ACOT2 functions as a serine esterase involved in lipid metabolism and undergoes acetylation and alternative splicing.

Experimental Guidance and Technical Tips

• For Western blot, expect a band around 53 kDa; validate with appropriate controls and sample preparation conditions.
• In IHC-P, optimize antigen retrieval and antibody dilution for your tissue type; a mitochondrial pattern is anticipated.
• The polyclonal antibody may recognize multiple epitopes; confirm specificity using knockout or knockdown samples if available.
• Cross-reactivity with human, mouse, and rat ACOT2 is indicated; verify in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials like this ACOT2 antibody, supporting mitochondrial fatty acid metabolism research. We offer optimized polyclonal and monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents to streamline your diagnostic development. For any rare-target sourcing needs, our team connects you with reliable solutions from concept to clinic.