Anti-Aconitase 2 (ACO2) Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q99798

Core Product Specifications and Parameters

Biological Background: Aconitase 2 (ACO2) Function and Localization

• ACO2 encodes mitochondrial aconitate hydratase, also referred to as citrate hydro-lyase, a key enzyme of the tricarboxylic acid (TCA) cycle.
• It catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate, playing a central role in cellular energy metabolism.
• The protein is localized to the mitochondrion and requires a [4Fe-4S] iron-sulfur cluster for its catalytic activity.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme function.
• ACO2 is a member of the aconitase family and contains a transit peptide that directs its import into mitochondria.
• Disease-associated variants in ACO2 are linked to neurodegenerative disorders, underscoring its importance in neuronal health.
• The protein has been identified in comprehensive proteomics studies, confirming its expression across multiple human tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 501-780 of human ACO2; consider using this recombinant fragment as a positive control in ELISA.
• For Western blotting, a band at approximately 85 kDa is expected; validate specificity using ACO2-knockout cell lysates or siRNA-mediated knockdown.
• In immunofluorescence and IHC-P, mitochondrial staining is anticipated; optimize fixation and permeabilization conditions for your sample type.
• The antibody cross-reacts with mouse and rat ACO2; verify performance in the relevant model system before large-scale studies.
• Because this is a polyclonal antibody, lot-to-lot variability may occur; include appropriate internal controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes streamlined access to high-quality IVD raw materials, including validated antibody pairs and bulk ancillary reagents. For mitochondrial metabolism and TCA cycle research, we supply a range of antibodies targeting enzymes such as ACO2, alongside technical support to accelerate your assay development. From monoclonal antibody optimization to rare target raw-material sourcing, CamelBio is your partner from concept to clinic.