Anti-Aconitase 2 (ACO2) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99798

Core Product Specifications and Parameters

Biological Background: Aconitase 2 (ACO2) Function and Localization

• ACO2 encodes mitochondrial aconitate hydratase, also known as citrate hydro-lyase, a key enzyme of the tricarboxylic acid (TCA) cycle.
• Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate, essential for cellular energy metabolism.
• Belongs to the aconitase family and features a 4Fe-4S iron-sulfur cluster critical for its catalytic activity.
• Requires iron as a cofactor; it is a metal-binding lyase.
• Localizes to the mitochondrial matrix, directed by an N-terminal transit peptide.
• Subject to post-translational modifications including acetylation and phosphorylation that may regulate function.
• Disease variants are linked to neurodegenerative disorders, consistent with aliases OCA8, OPA9, and ICRD.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–150 amino acids of human ACO2, which includes the mitochondrial transit peptide; detection may reflect precursor or mature forms depending on sample processing.
• For Western blotting, expect a band near 85 kDa; validate with known controls and consider potential shifts due to post-translational modifications.
• In IHC-P and IF/ICC, optimize antigen retrieval and confirm mitochondrial localization using organelle markers.
• Cross-reactivity with mouse and rat is indicated; check sequence homology for other species if extended applications are planned.
• ELISA can be employed for quantitative analysis or high-throughput screening of ACO2 levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, including monoclonal antibodies such as this anti-ACO2 rabbit mAb. Our portfolio covers targets in mitochondrial metabolism and neurodegenerative disease research, supported by options for bulk reagents and customized sourcing of rare targets. Partner with us to efficiently bridge your supply chain from concept to clinic.