Anti-Aconitase 1 (ACO1) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P21399

Core Product Specifications and Parameters

Biological Background: Aconitase 1 (ACO1) Function and Localization

• ACO1 gene encodes cytoplasmic aconitate hydratase, also known as iron regulatory protein 1 (IRP1) or IRE-BP1, a bifunctional enzyme that senses intracellular iron levels and switches between two distinct activities.
• As an aconitase, it catalyzes the isomerization of citrate to isocitrate via cis-aconitate in the cytoplasm when cellular iron is sufficient; this activity depends on the presence of a 4Fe-4S cluster.
• Under iron deprivation, ACO1 loses its iron-sulfur cluster and acquires mRNA-binding activity, interacting with iron-responsive elements (IREs) in untranslated regions of target mRNAs to post-transcriptionally regulate genes involved in iron uptake, storage, and utilization. Related references: PMID:1281544 | PMID:1946430 | PMID:8041788 | PMID:23891004
• The protein localizes predominantly to the cytoplasm and cytosol.
• ACO1 belongs to the aconitase/IPM isomerase family and is classified under keywords such as 4Fe-4S, iron-sulfur, lyase, tricarboxylic acid cycle, RNA-binding, and phosphoprotein.
• It is a known phosphoprotein and has been identified in numerous proteomics studies as a reference proteome component.
• Alternative names reflect its multifunctional role: IRP1, ACONS, HEL60, IREB1, IREBP, IREBP1.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide targeting amino acids 1-100 of human ACO1; consider verifying reactivity with the full-length native protein in your sample system.
• For Western blot, a band near 98 kDa is expected, though post-translational modifications or iron-sulfur cluster status may influence migration.
• When performing immunofluorescence (IF/ICC), validate appropriate fixation and permeabilization protocols for cytoplasmic antigens.
• Cross-reactivity with mouse and rat is stated; however, confirm performance in your specific experimental conditions due to potential interspecies variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, including high-performance monoclonal antibodies such as this ACO1/IRP1 rabbit mAb for iron metabolism research. We cater to diagnostic manufacturers and labs with validated antibody pairs, optimized monoclonals/polyclonals, bulk ancillary reagents, and custom sourcing for rare targets, supporting you from concept to clinic.