Anti-Aconitase 1 (ACO1) Polyclonal Antibody for WB - P21399

Core Product Specifications and Parameters

Biological Background: Aconitase 1 (ACO1) Function and Localization

• Aconitase 1 (ACO1), also known as IRP1 (Iron Regulatory Protein 1) or IRE-BP 1, is a bifunctional cytosolic protein that acts as an iron sensor.
• Under low iron conditions, ACO1 binds to iron-responsive elements (IREs) in mRNAs, regulating translation of ferritin and aminolevulinate synthase, and stabilizing transferrin receptor mRNA. Related references: PMID:1281544 PMID:1946430 PMID:23891004
• When iron is abundant, ACO1 assembles a 4Fe-4S cluster, which switches off its RNA-binding activity and activates its aconitase enzyme function, catalyzing the isomerization of citrate to isocitrate in the cytoplasm. Related references: PMID:1281544 PMID:1946430 PMID:8041788
• The protein is predominantly localized in the cytoplasm/cytosol, consistent with its role in iron homeostasis and citrate metabolism.
• Post-translational modifications include phosphorylation, and it belongs to the aconitase/IPM isomerase family, featuring a 4Fe-4S cluster.
• ACO1 is involved in the tricarboxylic acid cycle and cellular iron homeostasis, making it a relevant target in studies of metabolism and iron-related disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-170) of human ACO1, which may allow recognition of both the apo-form and holo-form; experimental validation is recommended.
• For Western blot, consider a starting dilution range of 1:500 to 1:2000 and optimize using appropriate positive controls (e.g., HeLa, HepG2, or tissue lysates from human, mouse, or rat). The predicted molecular weight is approximately 98 kDa; use an SDS-PAGE gel percentage suitable for high molecular weight proteins.
• For ELISA, titrate the antibody to determine optimal coating and detection conditions; ensure antigen integrity and buffer compatibility.
• Validate specificity in your model system by including negative controls (e.g., knockout or knockdown samples) or by pre-adsorption with the immunogen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop platform for diagnostic manufacturers, laboratories, and research institutes, offering a comprehensive portfolio of IVD raw materials. This ACO1 polyclonal antibody supports studies in iron metabolism and metabolic disorders. We supply validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare-target raw materials to streamline your assay development from concept to clinic.