Anti-Aconitase 1 (ACO1) Polyclonal Antibody for WB, ELISA - P21399

Core Product Specifications and Parameters

Biological Background: Aconitase 1 (ACO1) Function and Localization

• ACO1 encodes cytoplasmic aconitate hydratase, also known as iron regulatory protein 1 (IRP1), a bifunctional protein that serves as an iron sensor and regulates iron homeostasis.
• Under low cellular iron, IRP1 binds to iron-responsive elements (IREs) in the untranslated regions of target mRNAs, blocking translation of ferritin and aminolevulinic acid synthase while stabilizing transferrin receptor mRNA. Related references: PMID:1281544 PMID:1946430 PMID:23891004
• In iron-replete conditions, IRP1 assembles a 4Fe-4S cluster that abrogates RNA binding and confers aconitase activity, catalyzing the isomerization of citrate to isocitrate in the tricarboxylic acid cycle.
• ACO1 is primarily localized in the cytoplasm/cytosol.
• Post-translational modifications include phosphorylation, and the protein contains a critical iron-sulfur cluster.
• It plays a key role in cellular iron metabolism and the TCA cycle, with RNA-binding and lyase functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (aa 100–200), likely enabling cross-reactivity with mouse and rat ACO1; verify with species-specific controls.
• For Western blot, a band at approximately 98 kDa is expected; ensure the use of reducing conditions and appropriate molecular weight markers.
• Validate antibody specificity using ACO1 knockdown or knockout cell lines as negative controls.
• For ELISA, consider coating with recombinant ACO1 protein or using cell lysates with known expression levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw-material procurement, connecting diagnostic manufacturers with high-quality reagents like this anti-ACO1 antibody for iron metabolism and TCA cycle research. We offer a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. Trust CamelBio to streamline your development from concept to clinic with reliable, one-stop supply solutions.