Anti-ACLY Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P53396

Core Product Specifications and Parameters

Biological Background: ACLY Function and Localization

• ACLY, also known as ATP-citrate synthase or citrate cleavage enzyme, is a key metabolic enzyme connecting glucose metabolism to lipid synthesis.
• It catalyzes the conversion of citrate to oxaloacetate and acetyl-CoA in an ATP-dependent manner, with magnesium as a cofactor.
• The generated acetyl-CoA is essential for de novo lipogenesis, cholesterol biosynthesis, and histone/protein acetylation.
• ACLY functions as a homotetramer and is primarily located in the cytoplasm/cytosol.
• It undergoes various post-translational modifications, including phosphorylation and acetylation, which modulate its enzymatic activity and cellular functions.
• Dysregulation of ACLY is implicated in metabolic disorders, cancer, and cardiovascular diseases.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fragment spanning amino acids 752-1101 of human ACLY; it may detect both full-length and potential isoforms.
• For Western blot, expect a band around 121 kDa; optimize loading and blocking conditions for your specific lysates.
• In IHC-P, antigen retrieval protocols should be optimized; consider using liver or adipose tissue as positive controls.
• The antibody shows cross-reactivity with mouse and rat, enabling its use in rodent models; validate in your experimental system.
• For ELISA, a recombinant protein standard can assist in assessing sensitivity and linearity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibodies, including this anti-ACLY monoclonal antibody. With deep expertise in metabolism and disease research, we bridge the gap from concept to clinic by providing quality reagents, technical services, and customized solutions to accelerate your diagnostic development.