Anti-ACHE Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P22303

Core Product Specifications and Parameters

Biological Background: Acetylcholinesterase Function and Localization

• Acetylcholinesterase (ACHE) is a serine esterase that catalyzes the rapid hydrolysis of the neurotransmitter acetylcholine at cholinergic synapses, terminating synaptic transmission.
• The human ACHE gene encodes multiple isoforms via alternative splicing, including the erythrocytic isoform H, commonly referred to by aliases such as YT, ACEE, ARACHE, and N-ACHE.
• ACHE belongs to the cholinesterase family and plays a critical role in neuromuscular junction signaling and neuronal apoptosis regulation.
• Subcellularly, ACHE localizes to the synapse, cell membrane, nucleus, and is also secreted; it exists as both membrane-bound (GPI-anchored) and soluble forms.
• The protein undergoes post-translational modifications including disulfide bond formation and glycosylation, and carries blood group antigen determinants.
• Isoform H is highly expressed in erythrocytes, consistent with its anchored membrane form and role in acetylcholinesterase activity in blood.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 398-617) of human ACHE, which may be conserved across species; consider verifying sequence homology for mouse and rat targets.
• For Western blot (WB), the predicted molecular weight is ~68 kDa; include appropriate positive controls such as erythrocyte or neuronal lysates.
• In immunofluorescence/ICC, note that ACHE can localize to the cell membrane, nucleus, and is secreted; permeabilization and fixation conditions may need optimization.
• For ELISA applications, use recombinantly expressed ACHE or native protein from serum/plasma as capture antigen; validate with known standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a reliable, one-stop sourcing channel for high-quality IVD raw materials. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for targets such as acetylcholinesterase, supporting the development of assays for neurotransmission and neurotoxicity studies. Whether you need rare-target raw materials or scale-up quantities, CamelBio streamlines your supply chain from concept to clinic.