Anti-ACE2 Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - Q9BYF1

Core Product Specifications and Parameters

Biological Background: ACE2 Function and Localization

• ACE2 functions as a carboxypeptidase that cleaves angiotensin II to angiotensin 1-7, a vasodilator, and angiotensin I to angiotensin 1-9, regulating cardiovascular homeostasis. Related references: PMID:10924499, PMID:10969042, PMID:11815627, PMID:27217402
• It also processes other vasoactive peptides including apelin, casomorphins, and dynorphin A with high efficiency. Related references: PMID:11815627, PMID:27217402, PMID:28293165
• ACE2 acts as the host cell receptor for SARS-CoV and SARS-CoV-2, facilitating viral entry.
• ACE2 is expressed in endothelial cells, enterocytes, renal proximal tubule, heart, kidney, testis, and gastrointestinal system; in lung, it shows low and variable expression in alveolar type 2 cells. Related references: PMID:15141377, PMID:10924499, PMID:10969042, PMID:12459472, PMID:15231706, PMID:15671045, PMID:32170560, PMID:32715618, PMID:18424768
• Subcellularly, ACE2 is found at the cell membrane, secreted, in the cytoplasm, and at the apical membrane of polarized cells.
• The full-length protein is approximately 92 kDa, with a shorter 53 kDa isoform detectable, likely due to alternative splicing or proteolytic processing.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bonds, which may influence epitope accessibility.
• The C-terminal collectrin-like domain interacts with SLC6A19, regulating its surface expression. Related references: PMID:18424768, PMID:19185582

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 18-740; thus, the antibody may recognize both full-length and truncated isoforms. In WB, expect bands around 92 kDa (full-length) and potentially 53 kDa; validate with appropriate controls.
• For IF-P and IHC-P, membrane and cytoplasmic staining is anticipated; optimize fixation and permeabilization to preserve epitopes, especially for apical membrane detection in polarized cells.
• In ELISA, consider testing both direct and sandwich formats; the peptide immunogen may require specific coating conditions. Perform cross-reactivity testing against related ACE family members to confirm specificity.
• Given the role of ACE2 in SARS-CoV-2 entry, ensure biosafety level 2 or higher when handling potentially infected samples in application-based studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with a streamlined path from concept to clinic, offering one-stop access to premium IVD raw materials. Our Anti-ACE2 Rabbit PolymAb® supports cardiovascular and infectious disease research with validated performance in multiple applications. Beyond ACE2 antibodies, we supply matched antibody pairs, bulk ancillary reagents, and can assist in sourcing rare-target raw materials, all backed by technical and supply chain support to accelerate your IVD development.